ABF Files from Clampex 10.2 and Igor

Dan11
Posts: 1
Joined: 2011-10-28
Location: United States

I've fruitlessly searched the internet for an answer to this, so now I'm here to ask you fine people :)

My lab uses the Axon Systems suite, which includes obviously all the pClamp software. I'm currently using Clampex 10.2 as my data acquisition software, which saves files in what I think is ABF 2.X (I don't know if it's 2.0 or 2.1 or what). Please correct me if I'm wrong about that.

I need to import these files into Igor for analysis, but I'm having some difficulty. The "Load AxonBinary File" macro is not work, which I was expecting since it's only compatible to up to ABF 1.5 (as far as I know).

One of the labs I previously worked in I know converted the ABF files to another axon file format (whether this was an older format such as ABF 1.5 to allow the usage of the macro or some other type of format, I do not know) to allow it to be imported into Igor. I don't remember the procedure, though, and I'm pretty much out of ideas of how to get these files into Igor.

I know there's a way to import them, but I'm just unsure of how. Hopefully someone here can shed some light on the issue.

Thanks :)

Dan


[ last edited October 28, 2011 - 12:54 ]
Posts: 632
Joined: 2007-06-29
Location: United States

I think the Neuromatic package includes an ABF loader, at least on Windows. You can get it for free here: http://www.NeuroMatic.ThinkRandom.com/

If you're importing ABF files, you may find useful functionality in Neuromatic.

John Weeks
WaveMetrics, Inc.
support@wavemetrics.com


Sweetma
Posts: 1
Joined: 2011-12-02
Location: United States

Dan,

Any success? I am having the same problem.

I downloaded the neuromatic "loader." It runs, but Y=0 for all waves points.

Thanks,
Sweetma


[ last edited December 2, 2011 - 09:24 ]
Posts: 632
Joined: 2007-06-29
Location: United States

Sweetma wrote:
Any success? I am having the same problem.

I downloaded the neuromatic "loader." It runs, but Y=0 for all waves points.


Perhaps you should send an e-mail to Jason Rothman Jason@ThinkRandom.com about the problem. If the ABF file format has been updated, he may be able to modify his loader to accommodate it. He's generally pretty responsive to e-mails.

John Weeks
WaveMetrics, Inc.
support@wavemetrics.com


carlosro
carlosro's picture
Posts: 1
Joined: 2008-03-27
Location: United States

To convert Clampex 10 (.ABF 2.X ) files to Igor use this free program called Stimfit:

http://code.google.com/p/stimfit/

Load your .ABF file and export to Igor format from there. Multiple sweeps (I/V plot, for instance) are saved as Igor Multidimensional wave.
Best

Carlos Rozas
Columbia University


[ last edited January 15, 2012 - 11:52 ]

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