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automatic counting of spontaneous calcium activity transient peak/min or sec

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Manojshree October 18, 2010 - 09:31
Posts: 2
 Joined: 2010-10-18
 Location: United States

Dear Friends,

I am rather new to the use of Igor, so this may be a very nave question. I would appreciate advice on creating an efficient procedure for implementing it.

I am recording spontaneous calcium activity and I would like to count spontaneous calcium activity transient peak/min or sec. I would like to count it automatically like mini analysis did for patch clamp data. Here I would like to count it for several cells traces at a time and I was wondering if there is procedure to do it using Igor.

Many thanks for your help,
Manoj


[ last edited April 25, 2012 - 08:00 ]
johnweeks
 October 18, 2010 - 10:20
Posts: 836
 Joined: 2007-06-29
 Location: United States

You might want to look into the Neuromatic package, written by an Igor user who is a neuroscientist. Find it at http://www.NeuroMatic.ThinkRandom.com/. It has an optional module for doing data acquisition using either National Instruments or Instrutech (now HEKA) DAQ hardware.

John Weeks
WaveMetrics, Inc.
support@wavemetrics.com
RGerkin October 25, 2010 - 01:44
RGerkin's picture
Posts: 129
 Joined: 2008-02-22
 Location: United States

Neuromatic can do this (it does mini detection, which is basically the same thing). I also have a package for event detection like this, although a few changes might be in order for slow events like calcium transients. You can direct message me if you're interested.
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